Efficacy Studies

Study 1
Compared efficacy of deslorelin acetate at concentrations of 0.45, 0.9, 1.35 and 1.8 mg/mL with each group compared to placebo. There were 10 mares per treatment arm.

Treatment Group	Ovulation by 48 hours
Placebo	20%
SucroMate™ 1.0 mL	90%

Conclusion
The 1.0 mL SucroMate™ dose (1.8 mg/mL deslorelin acetate) was the most effective for advancing ovulation to within 48 hours following treatment.

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Study 2
A second study confirmed that 1.8 mg/mL SucroMate™ was more effective in advancing ovulation compared to a
0.9 mg/mL dose and a 1.35 mg/mL dose.

Treatment Group	Ovulation by 48 hours
Placebo	0% (0/12)
SucroMate™ 1.0 mL	82% (9/11)

Conclusion
The 1.0 mL SucroMate™ dose (1.8 mg/mL deslorelin acetate) was the most effective for advancing ovulation to within 48 hours following treatment. There was no detrimental effect on the cycle of administering SucroMate™ on 3 consecutive cycles.

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Study 3: Field Based Safety and Efficacy Study

A six site, blinded, controlled, multi-centered field efficacy study. Mares were randomly assigned into two groups: a single injection of either 1.0 mL of either saline or 1.0 mL SucroMate™ (1.8 mg/mL deslorelin acetate). Breeds included: 23 (8%) Standardbreds, 159 (56%) Thoroughbreds, 98 (35%) Quarter horses and 3 (1%) Paint horses. Route of administration: Intramuscularly into the fleshy part of the neck using an 18–21 gage needle. Parameters measured: percentage of mares ovulating within 48 hours following injection, as measured by ultrasound per rectum.

Time to ovulation was calculated from day of treatment to day of ovulation in 24hr increments Additional parameters measured: Percentage of mares that became pregnant following treatment and breeding in any ovulatory period. Mares were followed through gestation and all normal and abnormal deliveries were recorded. The number of estrus cycles required for mares to become pregnant was recorded. Injection sites were examined for signs of swelling, sensitivity to touch and skin temperature elevation daily for the first 7 days and then at 14 and 21 days post treatment.

Efficacy Results

Treatment Group	No. Cycles	No. Mares	Ovulated by 48 hours
Placebo	131	97	27%
SucroMate™	142	94	77%

Safety Results
There was no sensitivity to the touch nor increase in skin temperature observed at any injection site in either the placebo or SucroMate™ treated mares in any cycle.

Slight swelling occurred in the injection site in 3 mares in each treatment group on day 1 in Cycle 1 and 1 mare
on days 0 and 3 in Cycle 2.

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Additional Studies
Efficacy of Deslorelin Acetate (SucroMate) on Induction of Ovulation in American Quarter Horse Mares

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